Inhibition of adrenocortical cytochrome P-450scc by (20R)-20-phenyl-5-pregnene-3 beta,20-diol.
نویسندگان
چکیده
منابع مشابه
Studies on the Biosynthetic Conversion of Cholesterol into Pregnenolone SIDE CHAIN CLEAVAGE OF A t-BUTYL ANALOG OF ZOa-HYDROXYCHOLESTEROL, (20R)-20-t-BUTYL-3- PREGNENE-SD, 20-DIOL, A COMPOUND COMPLETELY
(20R)-20-f-Butyl-5-pregnene-3/3,20-diol, an analog of 20~ hydroxycholesterol, has been synthesized and its metabolism studied. Since C-22 in this synthetic compound is completely substituted, this position is unavailable for biological oxygenation. When injected intravenously into a rabbit, the t -butyl analog is metabolized to urinary pregnanediol. When incubated with sonicated mitochondria fr...
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The interaction between P-450czl and NADPH-cytochrome P-450 reductase, both purified from bovine adrenocortical microsomes, has been investigated in a reconstituted system with a nonionic detergent, Emulgen 913, by kinetic analysis and gel filtrations. Steady state kinetic data in progesterone 2 1-hydroxylation showed formation of an equimolar complex between the two enzyme proteins at low Emul...
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Some ZO-P-tolyl analogs of ZOa-hydroxycholesterol and cholesterol have been synthesized and tested as precursors of pregnenolone. As in the previously synthesized t-butyl analog, (20R)-20-f-butyl-5-pregnene-3~,20-diol. C-22 in these synthetic compounds is completely substituted and therefore unavailable for biological hydroxylation (1). (20R)-20-(&Tolyl)-5-pregnene-3P,20-diol, I, when incubated...
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In the title compound, C(26)H(24)N(2)O(2)Se, the selenadiazole ring is essentially planar [maximum deviation = 0.004 (3) Å]. The dihedral angle between the selenadiazole ring and the attached benzene ring is 50.17 (1)°. The crystal packing is stabilized by inter-molecular C-H⋯N inter-actions.
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The binding of adrenodoxin to cytochrome P-450scc and the intracomplex electron transfer from the iron-sulfur center to the heme have been studied. Salt sensitivity of the protein complex suggests the participation of electrostatic forces, as is also seen for the complex of adrenodoxin with NADPH-adrenodoxin reductase. Differences in ion specificities for the complexes of adrenodoxin with the o...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)32741-8